E. coli carbonic anhydrase - (can or YadF) have sequence, assay ok, expression?, essentiality?

http://ecogene.org/geneinfo.php?eg_id=EG12319

forms Synthetic lethal with cynT....




Organism: E. coli
Target: carbonic anhydrase- (can or YadF)

NCBI Gene # or RefSeq#:

EC#: 4.2.1.1

PDB # or closest PDB entry is using homology model:

1I6O- CRYSTAL STRUCTURE OF E. COLI BETA CARBONIC ANHYDRASE (ECCA)
1I6P
1T75

Background/Disease:

Image of protein (PyMol or etc):

http://www.pdb.org/pdb/explore/explore.do?structureId=1I6O

http://www.pdb.org/pdb/explore/explore.do?structureId=1I6P

http://www.pdb.org/pdb/explore/explore.do?structureId=1T75

CDS Gene Sequence:

ATGAAAGACATAGATACACTCATCAGCAACAATGCACTATGGTCAAAAATGCTGGTGGAAGAGGATCCCGGGTTTTTTGAGAAACTGGCACAAGCGCAAAAACCGCGCTTTCTATGGATTGGATGTTCCGACAGTCGCGTTCCTGCAGAACGTTTAACCGGTCTTGAGCCGGGCGAACTCTTTGTTCACCGTAATGTTGCTAACCTGGTCATTCACACTGACCTGAACTGCCTTTCCGTGGTTCAGTATGCAGTGGATGTACTCGAAGTTGAACACATTATTATCTGTGGCCACTACGGTTGCGGCGGCGTACAAGCCGCAGTTGAAAACCCGGAACTGGGGCTTATCAACAACTGGCTGCTGCATATCCGCGATATCTGGTTCAAACATAGCTCATTGCTCGGCGAAATGCCGCAAGAGCGCCGTCTGGATACCTTGTGTGAACTGAACGTCATGGAACAGGTGTATAACCTGGGCCACTCCACCATTATGCAATCAGCGTGGAAACGCGGGCAGAAAGTTACCATTCACGGCTGGGCCTACGGCATTCACGACGGCTTGCTGCGTGATCTGGATGTTACCGCCACCAACCGCGAAACCCTTGAGCAACGTTACCGTCACGGGATTTCCAACCTCAAGCTGAAACACGCCAACCACAAATAA

Amino Acid Sequence:
>EG12319 Can Carbonic anhydrase, beta class
MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSDSRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCGGVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGHSTIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHANHK

length of your protein in Amino Acids
220 aa

Molecular Weight of your protein in kiloDaltons using theExpasy ProtParamwebsite

25096.7 daltons = ~25 kDa


Primer Overlap Sequence for YadF:
ATGAAAGACATCGACACCCTCATCAGCAACAACGCGCTGTGGTCTAAAATGCTGGTTGAA GAAGACCCTGGTTTCTTCGAAAAACTGGCGCAGGCGCAGAAACCGCGTTTCCTGTGGATC GGTTGCTCTGACTCTCGTGTTCCGGCGGAACGTCTGACGGGTCTGGAACCGGGTGAACTG TTCGTTCACCGTAACGTTGCGAACCTGGTTATTCACACGGACCTGAACTGCCTGTCTGTT GTTCAATACGCGGTGGATGTTCTGGAAGTTGAACACATCATCATCTGCGGTCACTACGGT TGCGGTGGTGTTCAGGCGGCTGTTGAAAACCCGGAACTGGGTCTGATCAACAACTGGCTG CTGCACATCCGTGATATCTGGTTCAAACACTCTTCTCTGCTGGGTGAAATGCCGCAAGAA CGTCGCCTGGACACGCTCTGCGAACTGAACGTTATGGAACAGGTTTACAACCTGGGTCAC TCTACCATCATGCAGTCTGCGTGGAAACGTGGTCAGAAAGTTACCATCCACGGTTGGGCG TACGGTATTCACGACGGCCTGCTCCGCGACCTCGACGTCACTGCGACGAACCGCGAAACC CTGGAGCAGCGTTACCGTCACGGTATCTCTAATCTGAAACTGAAACACGCGAACCACAAA TAA

Primer design for pNIC-Bsa4 cloning:
Forward Primer: TACTTCCAATCCATGAAAGACATCGACACCCT

Reverse Primer: TATCCACCTTTACTGTTATTTGTGGTTCGCGTGT

E. coli carbonic anhydrase - cynT

http://ecogene.org/geneinfo.php?eg_id=EG10176

Organism: E. coli
Target:carbonic anhydrase - cynT
NCBI Gene # or RefSeq#:
EC#:4.2.1.1

PDB # or closest PDB entry is using homology model: N/A

Background/Disease:

Image of protein (PyMol or etc): N/A

CDS Gene Sequence:
>EG10176 cynT Carbonic anhydrase, beta class
GTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAACAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCGGGCAATATCGTCCCTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCATATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCAAACTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCCGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAA Amino Acid

Amino acide Sequence: >EG10176 CynT Carbonic anhydrase, beta classMKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRLVPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCGAMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANLQTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTAA

length of your protein in Amino Acids: 219 aa
Molecular Weight of protein: 23764.26 daltons
Primer overlap sequence of CynT from DNAworks:

ATGAAAGAAATCATCGACGGTTTCCTGAAATTCCAACGTGAAGCTTTCCCTAAACGCGAA GCGCTCTTCAAACAGCTGGCCACCCAGCAGTCTCCACGTACCCTGTTCATCTCTTGCTCT GACTCTCGTCTGGTACCGGAACTGGTTACCCAGCGTGAGCCGGGTGACCTGTTCGTTATC CGTAACGCGGGTAACATTGTTCCGTCTTACGGCCCAGAACCGGGTGGCGTTAGCGCGTCT GTTGAATACGCGGTTGCGGCTCTGCGTGTTTCTGACATCGTTATTTGCGGTCACTCTAAC TGCGGTGCGATGACCGCGATTGCCTCTTGCCAGTGCATGGACCACATGCCTGCAGTTTCT CACTGGCTGCGTTATGCGGATTCTGCACGTGTTGTTAATGAGGCGCGTCCACACTCTGAC CTGCCGTCTAAAGCGGCTGCGATGGTTCGTGAAAACGTTATTGCACAGCTCGCCAACCTG CAGACCCACCCGAGCGTTCGCCTGGCTCTGGAGGAAGGTCGTATCGCACTCCACGGTTGG GTTTACGACATCGAATCTGGTTCTATCGCGGCGTTCGATGGTGCGACCCGTCAGTTCGTT CCGCTGGCGGCGAACCCTCGTGTTTGTGCGATCCCTCTGCGCCAGCCGACTGCCGCGTAA

Primers for pNIC-Bsa4 cloning:
Forward primers: TACTTCCAATCCATGAAAGAAATCATCGACGG
Reverse primers: TATCCACCTTTACTGTTACGCGGCAGTCGGCTGG